Identification of early gene expression changes during human Th17 cell differentiation

Reference:

S. Tuomela, V. Salo S. K. Tripathi, Z. Chen, K. Laurila, T. Äijö, B. Gupta, L. Oikari, B. Stockinger, H. Lähdesmäki, and R. Lahesmaa. Identification of early gene expression changes during human th17 cell differentiation. Blood, to appear.

Abstract:

Th17 cells play an essential role in the pathogenesis of autoimmune and inflammatory diseases. Most of our current understanding on Th17 cell differentiation relies on studies carried out in mouse, whereas the molecular mechanisms controlling human Th17 cell differentiation are less well defined. In this study, we identified gene expression changes characterizing early stages of human Th17 cell differentiation through genome-wide gene expression profiling. CD4+ cells isolated from umbilical cord blood were used to determine detailed kinetics of gene expression after initiation of Th17 differentiation with IL1, IL6 and TGF. The differential expression of selected candidate genes was further validated at protein level and analyzed for specificity in initiation of Th17 as compared to initiation of other T helper subsets, namely Th1, Th2 and iTreg. This first genome-wide profiling of transcriptomics during the induction of human Th17 differentiation provides a starting point for defining gene regulatory networks and identifying new candidates regulating Th17 differentiation in human.

Suggested BibTeX entry:

@article{Tuomela2012:Th17,
    author = {S. Tuomela and V. Salo S. K. Tripathi and Z. Chen and K. Laurila and T. {\"A}ij{\"o} and B. Gupta and L. Oikari and B. Stockinger and H. L{\"a}hdesm{\"a}ki and R. Lahesmaa},
    journal = {Blood},
    language = {eng},
    title = {Identification of early gene expression changes during human Th17 cell differentiation},
    year = {to appear},
}